Completo HD (ITALIANO) Viol@ (1998)
Info:
Titolo originale: Viol@
Anno: 1998-09-11
Durata: 92 Minutes
Generi: Thriller
Lingua Originale: ITALIANO
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If any organic solvent, such as hexane, was added the iodine was readily transferred in the organic layer giving the layer a distinct color of iodine (7). Nitrite could also be detected by diazotizing sulfanilamide and coupling the product with N-(1-napthyl) ethylenediamine. Stealth Stealth is an adulterant which consists of two vials, one containing a powder (peroxidase) and another vial containing a liquid (hydrogen peroxide), both added to the urine. Stealth is capable of invalidating immunoassay screening of THC-COOH, LSD, and opiates using both Roche ONLINE assays and Microgenics CEDIA assays if these drug or metabolites are present in modest concentrations (125–150% of cutoff values). In addition, GC/MS confirmation could be affected (10). Valtier and Cody described a rapid spot test to detect the presence of Stealth in urine. Addition of 10 µL of urine to 50 µL of tetramethylbenzidine working solution, followed by addition of 500 µL of 0.1 M phosphate buffer solution caused the specimen to turn dark brown. Peroxidase activity could also be monitored by using a spectrophotometer (11). Our investigation showed that if a few drops of a urine specimen adulterated with Stealth were added to potassium dichromate followed by a few drops of 2N hydrochloric acid, a deep blue color developed immediately, which usually faded with time. Glutaraldehyde Glutaraldehyde containing products were one of the first that appeared in the market to invalidate drugs of abuse testing. Glutaraldehyde solutions are also available in hospitals and clinics as a cleaning agent. Glutaraldehyde at a concentration of 0.75% volume could lead to false-negative screening results for a cannabinoid test using the EMIT II drugs of abuse screen. Amphetamine, methadone, benzodiazepine, opiate, and cocaine metabolite tests are also affected at glutaraldehyde concentration between 1 and 2% using EMIT II immunoassays. Wu et al. described a simple fluorometric method for the detection of glutaraldehyde in urine. When 0.5 mL of urine was heated with 1.0 mL of 7.7 mmol/L potassium dihydrogen phosphate (pH 3.0) saturated with diethyl-thiobarbituric acid for 1 hour at 96–98°C in a heating block, a yellow green fluorophore developed if glutaraldehyde was present.
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